Figure 5.

Verification of the in vivo association of GmAGL15 with select DNA fragments. A, Fold enrichment calculations from qPCR on at least three independent ChIPs. Fold enrichment compares the amplicon of suspected target fragments recovered in immune ChIP and preimmune control ChIP. Results are normalized to the nonbound control fragment from GmTUB2. B, DSO calculations from qPCR on at least three independent ChIPs. Recovery of target fragments by coimmunoprecipitation with anti-AGL15 serum was compared with the recovery of a nonbound control DNA fragment (GmTUB2) in the same immune precipitation. Means and se are shown. Different letters indicate significant differences at P < 0.05. All DSOs for targets are significant. [See online article for color version of this figure.]