Figure 7.

Metabolic phenotype of SRT2 knockout line and simplified working model for SRT2 functions in plant mitochondria. A, Metabolite contents in srt2-1 compared with the wild type (WT; n = 5, ± se). The full list of metabolites including the absolute values can be found in Supplemental Table S5. Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test). B, SRT2 is dependent on NAD⁺ as substrate and interacts with the 76-kD subunit of complex I. SRT2 interacts and most likely deacetylates the ATP synthase (complex V) as well as a complex containing the AAC proteins AAC1 to AAC3, the putative di-and tricarboxylate transporter (DIC), and the metabolite transporter BOU. Lys acetylation sites of SRT2 interaction partners are indicated (AC). [See online article for color version of this figure.]