Figure 6.

Impact of HBI1 overexpression on BR biosynthesis. A, Expression of BR biosynthetic genes in 2-week-old seedlings measured by RT-qPCR. The transcript levels were normalized to U-box gene expression and then relative to time zero. Results are averages ± 2× se (n = 3). Two-way ANOVA/Holm-Sidak: **P < 0.01, ***P < 0.001. B, Endogenous BR levels of 4-week-old short-day-grown plants: typhasterol (TY), 6-deoxoCS, and CS. Results are averages from three independent biological repeats ± 2× se (n = 3). Two-way ANOVA/Holm-Sidak: **P < 0.01, *P < 0.05. All experiments were repeated at least three times with similar results.