Figure 7.

Mutation of the conserved residue Leu-214 impairs HBI1 function. A, Subcellular localization of HBI1-YFP in HBI1-ox 1 epidermal cells of 2-week-old seedlings. B, Phenotypes of long-day-grown 4-week-old Col-0 and a homozygous Arabidopsis line stably expressing 35S:HBI1(L214E)-YFP-HA [HBI1(L214E)-ox]. C, HBI1 expression levels in 2-week-old seedlings measured by RT-qPCR. The transcript levels were normalized to U-box gene expression and relative to Col-0. Results are averages ± 2× se (n = 3). One-way ANOVA/Holm-Sidak: ***P < 0.001. D, Hypocotyl lengths of 6-d-old seedlings grown in continuous light on one-half-strength MS medium. Results are averages ± 2× se across four independent experiments. Two-way ANOVA/Holm-Sidak: ^a≠bP < 0.001. E, Oxidative burst measured in leaf discs of 4-week-old plants in response to 100 nm elf18. Results are averages ± 2× se (n = 8). All experiments were repeated at least twice with similar results.