Figure 2.

SAP11_AYWB elicits the expression of Pi starvation-induced genes and increases the accumulation of Pi in Arabidopsis. A, The mRNA levels of genes triggered by Pi starvation responses in wild-type (Col-0) plants and 35S::SAP11_AYWB transgenic lines were examined by qRT-PCR and normalized to Actin2. The relative expression levels of each gene in wild-type plants were set at 1. B, The levels of Pi concentration in the aerial parts of the wild type and 35S::SAP11_AYWB transgenic lines grown in hydroponic solutions with 0.01 or 0.25 mm KH₂PO₄ were measured. Statistically significant differences were determined using Student’s t test (*P < 0.05, **P < 0.005 for transgenic plants versus the wild type).