Figure 7.
PHR1 is required for SAP11AYWB-triggered Pi starvation responses in Arabidopsis. A, Leaf morphologies of Arabidopsis transgenic plants overexpressing SAP11AYWB in the background of a phr1 mutant. The expression level of SAP11AYWB was detected by western blotting using specific antibodies against SAP11AYWB. Asterisk indicates the cross-reacting band appeared in all samples. Bar = 8 mm. B, The mRNA levels of genes involved in Pi starvation responses among wild-type (Col-0), phr1 mutant, and 35S::SAP11AYWB/phr1 transgenic plants were examined by qRT-PCR and normalized to Actin2. The relative expression levels of each gene in wild-type plants were set at 1. Statistically significant differences were determined using Student’s t test (*P < 0.05, **P < 0.005 for the wild type versus phr1; +P > 0.05 for phr1 versus 35S::SAP11AYWB/phr1).