Figure 8.

Real-time PCR analysis of PSR genes in AtERF070 RNAi and overexpression lines. Wild-type (WT), RNAi, and overexpression seedlings were germinated on one-half-strength MS medium for 7 d and then transferred to vertically oriented agar petri plates containing P+ and P− media for 1 week. Whole seedlings were harvested and used for real-time PCR analysis revealing the relative expression levels of PSR genes in the wild type and transgenics. The Arabidopsis housekeeping gene AtACTIN2 (At3g18780) was used as an internal control. Expression values for the PSR genes in wild-type P+ were set at 1. The values presented are means of three technical replicates ± se. Different letters indicate significant differences.