Figure 9.

Real-time PCR analysis of GA biosynthetic genes in AtERF070 RNAi and overexpression lines. Wild-type (WT), RNAi, and overexpression seedlings were germinated on one-half-strength MS medium for 7 d and then transferred to vertically oriented agar petri plates containing P+ and P− media for 1 week. Whole seedlings were harvested and used for real-time PCR analysis revealing the relative expression levels of GA biosynthetic genes in the wild type and transgenics. The Arabidopsis housekeeping gene AtACTIN2 (At3g18780) was used as an internal control. Expression values for the GA biosynthetic genes in wild-type P+ were normalized to 1. Different letters indicate significant differences in the relative level of gene expression. The values presented are means of three technical replicates ± se.