Figure 6.

Phenotypic effects of BSL2 overexpression. A, BES1 phosphorylation in 12-d-old seedlings of the indicated genotypes treated with 1 μm eBL or with the equivalent amount of solvent for 2 h in the light. bsu1-D (in a BRI1 background) is compared with its wild type (Wassilewskija-2 [Ws-2]). PRX, Peroxiredoxin. B, Response of 4-d-old etiolated Col-0 (black squares), BSL2:HA (white triangles), and BSL2:GFP (white squares) seedlings to Brz treatment. Data were compared at each concentration by one-way ANOVA; where significant differences were found, a post hoc Bonferroni test was performed. Asterisks indicate significant differences (***P < 0.001). Data are averages ± se of three independent experiments, with n > 15 seedlings. C, Effect of BSL2 overexpression on 45-d-old adult plants of the indicated genotypes. Bar = 5 cm. D, Effect of BSL2 overexpression on 45-d-old adult plants of the indicated genotypes. Bar = 5 cm. E, BES1 phosphorylation in the bri1-301 combinations shown in C. F, BES1 phosphorylation in the bin2-1 combinations shown in D. Western blots were processed as described in “Materials and Methods.” In E and F, G denotes BSL2:GFP and H denotes BSL2:HA. [See online article for color version of this figure.]