Figure 7.
Interaction between BSL and BR signaling intermediates. A to C, The interaction between the BSL phosphatases and BSK1 (A), BSK3 (B), and CDL1 (C) was assessed in Saccharomyces cerevisiae two-hybrid assays. Representative colonies were spotted on selective medium with increasing stringency. AD, Activation domain; 3AT, 3-amino-1,2,4-triazole; BD, DNA-binding domain; SD, synthetic dextrose. D, BSU1, but not BSL1 or BSL2, accelerates the turnover of BIN2 when coexpressed in N. benthamiana leaves. The asterisk marks BIN2:HA. The top gel was probed with anti-HA antiserum, and the bottom gel was probed with anti-TyrP-GSK3 monoclonal antibody.