Figure 8.

Effects of the simultaneous loss of function of BSL1, BSL2, and BSL3. A, Comparison between 3-week-old bsl1-2^−/− bsl2-2^+/− bsl3-1^−/− (left) and bsl1-2^−/− (right) plants. Bar = 5 cm. B, Cleared mature siliques of the indicated genotypes. Bar = 5 mm. Table 1, The progeny of the indicated genotypes carrying the segregating mutant allele significantly departed from the expected 3:1 ratio. Observed frequencies are as follows: 51% for bsl1-1^−/− bsl2-2^−/− bsl3-2^+/− and 52% for bsl1-2^−/− bsl2-2^+/− bsl3-1^−/−. Table 2, The progeny of the indicated genotypes significantly departed from the expected 9:3:3:1 ratio for two unlinked loci and fully viable gametes (F^obs versus F^exp). When the proportion of female gametes is corrected as 0.33 (BSL1 BSL2):0.33 (bsl1 BSL2):0.225 (BSL1 bsl2):0.115 (bsl1 bsl2), while the proportion of male gametes is kept at 0.25 for each genotype, the frequency distributions are not significantly different (F^obs versus F^{exp corr}). bsl1 and bsl2 indicate the presence of the T-DNA insertions. Data were compared with a χ² test. [See online article for color version of this figure.]