Table 2. Independent predictive factors of disease-free survival (DFS) and overall survival (OS) by multivariate analysis (Cox proportional hazards model).

Variables	Test set				Validation set
	DFS		OS		DFS		OS
	HR (95% CI)	P value	HR (95% CI)	P value	HR (95% CI)	P value	HR (95% CI)	P value
PTK7 expression
Negative vs Positive	2.319 (1.175–4.580)	0.015	1.801 (1.040–3.118)	0.036	3.304 (1.749–6.244)	<0.001	2.498 (1.298–4.807)	0.006
Tumor size (cm)
≤5 vs >5	1.875 (1.102–3.191)	0.021	1.832 (1.055–3.179)	0.031	1.496 (1.277–1.889)	0.018	2.081 (1.166–3.713)	0.013
Neural invasion
Absent vs Present	1.249 (0.631–2.473)	0.208	1.649 (0.821–3.310)	0.16	1.039 (0.631–1.710)	0.881	1.396 (0.834–2.339)	0.205
Angiolymphatic invasion
Absent vs Present	2.04 (1.200–3.470)	0.008	1.809 (1.038–3.155)	0.037	2.246 (1.190–4.241)	0.013	1.870 (0.999–3.497)	0.050
IHM
Absent vs Present	0.543 (0.243–1.209)	0.135	0.800 (0.351–1.826)	0.597	1.384 (0.856–2.239)	0.185	1.436 (0.858–2.402)	0.168
EHE
Absent vs Present	0.982 (0.568–1.698)	0.947	0.738 (0.414–1.315)	0.302	0.721 (0.423–1.229)	0.229	1.389 (0.840–2.295)	0.200
pT stage (7th AJCC)
T1 & T2 vs T3 & T4	2.319 (0.756–3.475)	0.523	1.216 (0.626–2.360)	0.563	1.769 (1.027–3.047)	0.040	1.855 (1.057–3.258)	0.031

Notes: HR, hazard ratio; CI, confidential interval; DFS, disease-free survival; OS, overall survival; IHM, intrahepatic metastasis; EHE, extrahepatic extension. All experiments were replicated thrice with triplicate repeated measures within each replication for each time point.