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. 2014 Feb 28;9(2):e90371. doi: 10.1371/journal.pone.0090371

Figure 2. A role for mDia2 in chemotactic migration in OVCA429 cells.

Figure 2

A. Transfected OVCA429 migrated for 5% FBS. Live single-cell tracking of transfected cells was performed, and total distance migrated calculated. At least 10 cells were tracked per condition. p values listed are calculated relative to respective empty vector controls. B. OVCA429 cells were transfected with lipid only, control siRNA or siRNA directed against mDia2 at increasing concentrations (25, 50, 100 nM, denoted by wedges) for either 72 or 96 h. mDia2 depletion was confirmed by Western blot. C. OVCA429 cells were transfected with 100 nM siRNA for 72 h, and transwell migration assays performed for 5 h towards a SFM or 10% FBS. The assay was performed in triplicate wells and the experiment was repeated thrice. A representative experiment is shown. p value shown are 10% FBS treated control vs. mDia2-depleted cells. All error bars correspond to SDs for that representative experiment.