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. 2014 Feb 28;9(2):e90486. doi: 10.1371/journal.pone.0090486

Figure 4. The tufA499 mutation is associated with a reduced expression of four tRNA aminoacyl synthetases.

Figure 4

Expression levels of the synthetase genes thrS, cysS, valS and proS were measured by quantitative real-time PCR in wild-type TH7507 (tufA+) and TH7509 (tufA499). Values are averages of six independent replicates and normalized to the wild-type levels. Standard deviations represented as error bars. The differences between the mRNA levels in the wild-type and the tufA499 strains are statistically significant according to an unpaired t-test, thrS: p = 0.0005, cysS: p = 0.0105, valS: p = 0.0225, proS: p = 0.0169.