. 2014 Feb 28;9(2):e90486. doi: 10.1371/journal.pone.0090486

Table 1. Bacterial strains.

Strain	Genotype
TH673	metA22 metE551 galE496 rpsL120 xyl-404 (Fels2-) Hlnb nml- H2 enx hsdL6 hsdA29 ilv proB1657::Tn10 srl-203::Tn10d-Cam recA1
TH4527	Salmonella enterica subsp. enterica serovar Typhimurium strain LT2 wild-type
TH7480	tufB::FRT^a trpE91/F′128 pro ⁺ lac ⁺ zzf-1831::Tn10dspc
TH7483	tufA499 tufB::FRT^a trpE91/F′128 pro ⁺ lac ⁺ zzf-1831::Tn10dspc
TH7507	tufB::FRT^a trpE91
TH7509	tufA499 tufB::FRT^a trpE91
TH7960	tufB::FRT^a trpE91/pBAD TOPO (spoT)
TH7963	tufA499 tufB::FRT^a trpE91/pBAD TOPO (empty vector)
TH7964	tufA499 tufB::FRT^a trpE91/pBAD TOPO (spoT)
TH7965	tufA499 tufB::FRT^a trpE91/pBAD TOPO (spoU)
TH7966	tufA499 tufB::FRT^a trpE91/pBAD TOPO (recG)
TH7975	tufA499 tufB::FRT^a trpE91 relA21::Tn10
TH7976	tufB::FRT^a trpE91 relA21::Tn10
TH7991	tufA499 tufB::FRT^a trpE91/pBAD TOPO (csgD 70 nt fragment)
TH8132	tufB::FRT^a trpE91 dksA::KanR
TH8133	tufA499 tufB::FRT^a trpE91 dksA::KanR
TH8385	tufA499 tufB::FRT^a trpE91 relA21::Tn10/pBAD TOPO (spoT)
TH8386	tufB::FRT^a trpE91 relA21::Tn10/pBAD TOPO (spoT)
TH8634	tufA499 tufB::FRT^a trpE91 relA21::Tn10/F′128 pro ⁺ lac ⁺ zzf-1831::Tn10dspc
TH8635	tufB::FRT^a trpE91 relA21::Tn10/F′128 pro ⁺ lac ⁺ zzf-1831::Tn10dspc

The coding sequence of tufB was precisely replaced with an FRT sequence by λ-Red-mediated chromosome recombineering [43], [44].