Figure 6. Treatment with L-Kynurenine fails to inhibit in vivo T_CD8 responses to T Ag.

WT mice were treated with L-Kyn (30 mg total as described in Materials and Methods) or PBS, and injected i.p. with C57SV cells. Nine days later, splenic T_CD8 were examined for IFN-γ accumulation following restimulation with C57SV cells or synthetic peptides corresponding to T Ag epitopes. T Ag-specific T_CD8 frequencies were determined after subtracting background and expressed as mean ± SEM of 6 L-Kyn-treated and 8 vehicle-treated mice (A). These values were used to calculate the absolute number of T Ag-specific T_CD8 present within each spleen (B).