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Cells were transfected with control siRNA, or vehicle control (LP2000), or AFP-siRNA for 48 hours. Apoptosis was detected by TUNEL assay. Cells were viewed under a phase contrast (A, C, E) and confocal microscopy (B, D, F). Compared with negative control group (A and B) and LP2000 group (C and D), cells transfected with AFP-siRNA (E and F) showed abundant apoptosis.
