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. 2014 Feb 28;9(2):e90660. doi: 10.1371/journal.pone.0090660

Figure 4. Effect of AFP silencing on the expression of MMP-9 and MMP-2, and the invasive ability of Huh-7 cells.

Figure 4

Cells were transfected with control siRNA, vehicle control (LP2000) and AFP-siRNA. The expression of MMP-9 and MMP-2 was examined by Western blot (A) and Real-Time PCR (E). Impact of AFP-siRNA silencing on the invasive ability of Huh-7 cells was examined by chamber invasion assay. Transfection of Huh-7 cells with AFP-siRNA led to a decreased cell invasion (D) compared to cells transfected with control siRNA (B) or LP2000 (C).