The beneficial effects of GUO were evaluated by a cylinder test. (A) Cylinder Test on 0, 1, 3, 7 and 15 days post-ischemia. GUO i.p. administration was performed 4 times (30 min before ischemia and 1 h, 3 h and 6 h after ischemia). *represents P<0.001 when comparing ischemia saline vs. sham saline and #represents P<0.001 when comparing ischemia saline vs. ischemia GUO 30 mg/Kg. n = 9–10 per group. (B) Cylinder Test on 0, 1, 3, 7 and 15 days post-ischemia. GUO i.p. administration was performed 4 times (immediately, 1 h, 3 h and 6 h after ischemia). *represents P<0.001 when comparing ischemia saline vs. sham saline, #represents P<0.01 when comparing ischemia saline vs. ischemia GUO 60 mg/Kg and &represents P<0.01 when comparing ischemia saline vs. Ischemia GUO 120 mg/Kg. n = 9–13 per group.