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. 2014 Feb 28;9(2):e90095. doi: 10.1371/journal.pone.0090095

Figure 4. Mechanism of Action Enzymatic Assay Results.

Figure 4

(A) Peptide dose response curve in the EZH2 HMT assay using 100 nM (10× enzyme) and 10 nM (1×enzyme) of reconstituted, recombinant PRC2. (B) Peptide dose response curve in the EZH2 HMT assay using 2.4 µM (10× SAM) and 0.24 µM (1× SAM) of 3H-labeled SAM. (C) Peptide dose response curve in the EZH2 HMT assay using 60 µM (10× substrate) and 6 µM (1× substrate) of H3K27me1.