Fig. 6.

The Mcl-1/Noxa axis plays a critical role in R-roscovitine and TRAIL-induced apoptosis in MZ-294 monolayer cells. a MZ-294 cells were treated with R-roscovitine (20 μM), TRAIL (100 ng/ml) and R-roscovitine (20 μM) + TRAIL (100 ng/ml) for 24 h and the expression of Mcl-1 and Noxa was assessed by western blot analysis. Actin was used as a loading control. b Images (scale bar = 20 μM) depicting transfection efficiency of the GFP-expressing scramble control and Noxa knockdown shRNA in MZ-294 cells and the subsequent knockdown of Noxa after 72 h are shown. c MZ-294 cells were transfected with a GFP-expressing Noxa knockdown shRNA and treated with R-roscovitine (20 μM) or R-roscovitine (20 μM) + TRAIL (100 ng/ml) for 48 h. The percentage of apoptotic GFP⁺ cells was determined by Hoechst staining. Data is expressed as mean ± SEM, **p < 0.01; ***p < 0.001 versus control cells; ⁺ p<0.05 versus R-roscovitine treated cells; ⁺⁺⁺ p < 0.001 versus R-roscovitine + TRAIL treated cells; data are from three independent experiments