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Eight transcription factors induced Best1::GFP+ colonies from human fibroblasts. (A) Morphological changes of HFF during the process of RPE lineage conversion. Best1::GFP+ cells exhibited cobblestone-like morphology at day 35. All scale bars: 100 μm. (B) Effects of individual factor withdrawal from 8 transcription factors (8TFs) on Best1::GFP+ colony formation. HFF cells transduced with mCherry were served as the control. Data are presented as mean ± s.d. of three replicates
