FIG. 5.
Inhibition studies of cell surface binding of SUWT-HA and SUHV3-HA glycoproteins. (A) Cell culture supernatants containing the soluble SUWT-HA protein were incubated with HeLa cells pretreated with either 100 μg/ml AMD3100 or 10 μg/ml anti-CXCR4 12G5 MAb. (B) The soluble SUHV3-HA was incubated with HeLa cells treated with 100 μg/ml AMD3100. (C) To test the ability of the anti-HIV-1 V3 MAb to interfere with the cell surface binding of the SU-HA glycoproteins, the SUWT-HA and SUHV3-HA proteins were first incubated with this MAb and then used in the binding assays as described in Materials and Methods. Input: One-tenth of the total amount of SUWT-HA or SUHV3-HA used in each binding reaction was immunoblotted with the anti-HA MAb.