FIG. 6.

(A) Synthesis and processing of the FIV Env glycoproteins. 293T cells expressing the wild-type (WT), ΔV3, or HV3 glycoproteins were lysed and viral proteins were resolved by SDS-PAGE and immunoblotted with the polyclonal serum against the FIV TM. The mobilities of the Env precursor (Pre) and TM subunit are indicated as are those of the molecular weight markers (in kDa). (B) Relative cell surface levels of mutant ΔV3 and chimeric HV3 glycoproteins as percentages of those of wild-type Env (WT) considered as 100%. The surface levels in transfected 293T cells of wild-type, ΔV3, or HV3 glycoproteins were quantitated by cellular ELISA as described in Materials and Methods.