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Chemically induced ASCs successfully underwent osteogenesis over 21 days. Absorbance values for eluted Alizarin Red S dye indicated that induced samples deposited thrice more calcified matrix than control samples (A, **p<0.0001). Induced (B) and control (C) ASCs stained with Alizarin Red S for 30 min showed clear, qualitative differences in calcified matrix deposition. Mean±standard deviation. Scale bars: 100 μm. Color images available online at www.liebertpub.com/tea
