Table 1.

Molecular Beacon and Target Sequences for Reference and Osteogenic mRNA

Gene	Beacon sequence 5′→3′
GAPDH27	CGACGGAGTCCTTCCACGATACCACGTCG
ALPL	CGCTCCAGAGTGTCTTCCGAGGAGGTCAAGGAGCG
ALPL target	TTGACCTCCTCGGAAGACACTCT

Stem regions are underlined. Remainder of oligonucleotide forms the loop region, which is complementary to the gene of interest. A 6-FAM fluorophore and BHQ1 quencher were attached to 5′ and 3′ ends, respectively. ALPL target is the reverse complement of the loop region of the ALPL beacon.