The expression profiles of receptors for epidermal growth factor (EGF), brain-derived neurotrophic factor (BDNF), and insulin-like growth factor-1 (IGF-1) in human r2PN zygotes (n=30). EGF, BDNF, and IGF-1 receptors (IGF-1Rs) were detected in the membranes of human r2PN zygotes. (A) EGF receptor (EGFR). (B) IGF-1 receptor (IGF-1R). (C) BDNF receptor (TrkB). (A1–A3) Show immunofluorescence for the three different receptors, (B1–B3) show propidium iodide nuclear staining and those in (C1–C3) show the merged images. Scale bar=25 μm.