FIG. 5.

Derivation of human embryonic stem cell (hESC) lines from r2PN blastocysts cultured with or without the EGF–BDNF–IGF-1 combination. (A) Derivation efficiency, including primary colony formation and ESC line establishment, was significantly increased when the culture medium was supplemented with EGF–BDNF–IGF-1. (B) Expanded blastocysts in r2PN cultured with EGF–BDNF–IGF-1. (C) Primary colony at day 7 after inner cell mass was implanted onto the feeder layers. (D) Typical hESC colony with distinct boundary, high nuclear/cytoplasm ratio, and tightly packed together. (E) Positive AP activity for hESC colony. Scale bar=50 μm.