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. 2013 Nov 21;23(6):563–575. doi: 10.1089/scd.2013.0420

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 8. — Evaluation of in vitro and in vivo differentiation in hESC by embryoid body (EB) and teratoma formation. (A) Typical EBs with round and smooth morphology were formed when the r2PN hESCs were cultured in suspension. The EBs expressed TUJ1 (ectoderm), SMA (mesoderm), and AFP (endoderm). (B) Genes from the endoderm (AFP), mesoderm (HBZ), and ectoderm (NEUROD) were identified in differentiated EB clumps. (C) Tissues from the three embryonic germ layers were identified in teratomas, including neuroectoderm (ectoderm), cartilage (mesoderm), and glandular tissue (endoderm). Scale bar=100 μm.