FIG. 1.

TuJ1, a neuron-specific β-3 Tubulin antibody (green) and Dulbecco's modified Eagle's medium (nuclear, blue) immunostaining of dorsal root ganglion (DRG) (A–D) and hippocampal cells (E–H) at 5 days in vitro (DIV) on substrates of glass coverslips (A, E), aligned polycaprolactone (PCL) (B, F), aligned small diameter polystyrene (PS) scaffolds (C, G), and aligned large diameter PS scaffolds (D, H) (scale bar 200 μm). In each case, cells on glass coverslips sent out neurites in random orientation. While DRG cells sent out neurites along fibers, hippocampal cells sent out neurites both parallel and perpendicular to fibers. Direction histograms for DRG on coverslips (I) and thin PS fibers (J), and hippocampal cells on coverslips (K) and thin PS fibers (L) have been included. Arrows show the direction of fiber alignment.