Table 2.
PCR oligonucleotide sequences used in this study
| Gene | Primer sequence | Product size (bp) | GeneBank |
|---|---|---|---|
| chicken-β-actin |
ctgaaccccaaagccaacag, ccagatccagacggaggatg |
214 |
X00182.1 |
| chicken VEGF |
gcagagcgcggagttgtc, gtccaccagggtctcaattgtc |
127 |
NM_001110355 |
| chicken GATA4 |
cggcctctcttgtgccaact, gatggacctgctggcgtctt |
203 |
U11887.1 |
| chicken Nkx2.5 |
accccgcgtcctcttttctc, cccaccatctccagggtctg |
197 |
NM_205164.1 |
| chicken TGF-β2 |
cagtgggaagaccccacatc, tgaatccatttccagccaag |
185 |
X59080.1 |
| chicken FN1 |
cgcccctaccactctgacac, cagctctgcaacgtcctcct |
175 |
XM_421868.2 |
| chicken NEO |
tccggatagctgccatgact, tggagtccagctcaccacaa |
161 |
U07644.1 |
| chicken HoxB1 | aacctctcgccttccctaaa, agctgcttggtggtgaagtt | 191 | NM_001080859 |