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. Author manuscript; available in PMC: 2015 Jan 16.
Published in final edited form as: Cell Rep. 2013 Dec 27;6(1):93–103. doi: 10.1016/j.celrep.2013.12.004

Figure 1. Identification and Mapping of the ky850 Mutation.

Figure 1

(A) Image and schematic of a wild-type zdIs5(Pmec-4::GFP) larval-stage four (L4) animal, illustrating the six mechanosensory neurons (PLML/R, PVM, ALML/R, and AVM). Anterior is left and ventral is down in this and all subsequent images.

(B) Image and schematic of a typical intact PLM neuron from a 3-day-old adult wild-type animal (B1) and that of a typical axon break (bracketed region) in a 3-day-old adult ky850 mutant (B2).

(C) Axonal breaks quantified in mec-17(ok2109) animals of progressive ages. Similar results were obtained for mec-17(u265) animals (data not shown). DOA, day-old adult.

(D) Penetrance of axonal breaks in ky850, QH4387 (3x outcrossed ky850), and mec-17(ok2109) animals, carrying the Pmec-4::mec-17 rescue transgene versus nontransgenic siblings.

(E) Penetrance of axonal degeneration in ky850 (black bar) animals and the different mec-17 alleles (gray bars). F1 cross progeny (white bars) were analyzed for phenotypic complementation. The scale bars represent 25 μm; error bars represent SE of proportion; n ≥ 100 animals; ***p < 0.001.