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. Author manuscript; available in PMC: 2015 Jan 16.
Published in final edited form as: Cell Rep. 2013 Dec 27;6(1):93–103. doi: 10.1016/j.celrep.2013.12.004

Figure 3. mec-17 Animals Display Aberrant Axonal Transport.

Figure 3

(A and B) Image and schematic of UNC-104/ kinesin-3 in PLM of wild-type (A) and mec-17(ok2109) (B) 3-day-old adult animals used to monitor axonal transport. UNC-104 was visualized using the jsIs1111[Pmec-4::unc-104::GFP] transgene. Insets show a magnified view of the distal axon tips. Arrowheads point to the distal tip of the PLM axon. The scale bars represent 25 μm.

(C) Quantification of UNC-104::GFP localization in PLM in wild-type and mec-17(ok2109) animals. Anterior neurite defined as per image shown in (A), with anterior localization and accumulation at presynaptic sites. Gray bars indicate additional localization in the PLM posterior neurite. White bars designate localization in both the anterior and posterior neurites with no presynaptic accumulation.

(D) Comparison of the number of animals displaying UNC-104::GFP pooling at the distal end of the PLM axon.

(E) Accumulation of UNC-104::GFP measured in different regions along the PLM axon in wild-type and mec-17(ok2109) animals at the L4 stage and in 3-day-old adults. This analysis excludes pooling at the distal tip.

The error bars represent SE of proportion; *p < 0.05; ***p < 0.001; n ≥ 51 animals.