Skip to main content
. Author manuscript; available in PMC: 2015 Jan 14.
Published in final edited form as: Biochemistry. 2013 Dec 20;53(1):127–134. doi: 10.1021/bi4012995

Figure 2.

Figure 2

Effect of 1D4-antibody on rhodopsin-catalyzed activation of Gt. Activation of Gt was monitored by following the binding of [35S]-GTPγS with time in the presence (diamonds) or absence (circles) of 1 µM 1D4-antibody using a filter-binding assay as described in the Experimental Procedures. Each reaction contained 5 nM of the rhodopsin triple mutant in Nanodiscs reconstituted with 11-cis-retinal, 1 µM Gt, and 3 µM GTP. Solid symbols, reaction in dark; open symbols, reaction after to exposure to light (300 W tungsten bulb for 30 sec at t = 6.5 min). Residual 1D4-peptide was present at a concentration 20-fold lower than that of the antibody combining sites.