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Expression of signaling molecules on breast cancer cells in presence or absence of γδ T cells. Breast cancer cells were cocultured with γδ T cells for 24 hr at 1:5 ratios. Total proteins were isolated from cancer cells after gentle removal of γδ T cells, and Western blot was performed for various signaling molecules stated in SkBr7 (left panel) and BrCa-MZ01 (right panel) cells. Each experiment was performed at least three times using γδ T cells from three different donors.
