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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Cell Microbiol. 2013 Sep 19;16(1):131–145. doi: 10.1111/cmi.12211

Figure 4. Activation of MAPK and NF-κB pathways in SAS cells incubated with P. gingivalis.

Figure 4

Figure 4

SAS cells were incubated with P. gingivalis at an MOI of 1 for the indicated times, then the lysates were subjected to immunoblotting. Blots showing phosphorylation and total proteins are expressed from a densitometric analysis with arbitrary units. β-actin was included as a loading control for whole cell lysates. Nucleolin was included as a loading control for the nuclei. Tubulin was included as a loading control for cytoplasm. Data shown are representative of three independent experiments. (A) MAPK pathways, (B) IκBα, and (C) NFκB in nuclear and cytoplasmic fractions. Densitometric analysis of blots showing phosphorylation and total proteins, expressed in arbitrary units. β-actin was included as a loading control. Data are shown as the mean ± SD of three independent experiments and were analyzed with a t test.