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. 2014 Mar 3;5:80. doi: 10.3389/fmicb.2014.00080

Figure 6.

Figure 6

Conserved regions of amino acids sequences of rdhA genes. (A) Regions conserved among experimentally characterized reductive dehalogenases. The members are indicated by their name and protein accession. A label in the parentheses indicates the cluster name as in Figures 4, 5. Amino acid residues are colored according to the similarity of their physico-chemical properties. Conserved regions and two Fe-S binding motifs are labeled as C1 through C5 and FeS (FeS1 and FeS2), respectively. Consensus residues are those conserved in ≥80% (10 or more among 13 enzymes); highlighted residues are those conserved in ≥60% (7 or more among 13). Experimental evidences have been reported for the following proteins: TceA.YP_180831.1 (Magnuson et al., 1998), VcrA.AAQ94119.1 (Müller et al., 2004), DcpA.AGS15112.1, CbrA.CAI82345.1 (Padilla-Crespo et al., 2014), CprA.AAD44542.1 (van de Pas et al., 1999; Smidt et al., 2000), PceA.CAD28790.2 (Maillard et al., 2003), PceA.AAC60788.1 (Neumann et al., 1998), Ssed_3769.YP_001475501.1 (Lohner and Spormann, 2013), CfrA.AFQ20272.1 (Tang and Edwards, 2013), BhbA.AFV28965.1 (Chen et al., 2013). Experimental support (transcription under organohalide compounds) has been reported for the following proteins: BvcA.AAT64888.1 (Krajmalnik-Brown et al., 2004). MbrA.ADF96893.1 (Chow et al., 2010), PrdA.BAE45338.1 (Tsukagoshi et al., 2006). (B) Residues of the rdhA-homologous genes in the sediment samples around the conserved regions defined in (A). The members are indicated by locus tag. A label in the parentheses indicates the cluster name as in Figures 4, 5. Double slash characters “//” denotes truncation at the end of the nucleotide sequence.