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. 2014 Mar 3;8:66. doi: 10.3389/fncel.2014.00066

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Copyright © 2014 Poon, Goh, Voorhoeve and Fivaz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantifying the impact of miRNAs on endogenous synapses in mature hippocampal neurons. Hippocampal neurons were transduced with viruses expressing the empty vector (CT), miR-196a, or miR-27b, and immunostained (DIV21) against pre- and post-synaptic markers. (A–C) Raw confocal images (63×) of DIV21 hippocampal neurons. Scale bar, 5 μm. Inset, magnified view of boxed area. (D) Merged image of SV protein synaptobrevin (green) and post-synaptic protein homer (purple). (E) Output image of our synapse detection algorithm showing the neuronal outline (red) and synapses (white puncta). (F–H) Representative images of synapses detected in dendrites of neurons transduced with an empty vector (F), miR-196a (G), or miR-27b (H). Scale bar, 5 μm. (I) Quantification of synaptic density. n = 3. ^**p < 0.01, ANOVA.