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. 2014 Mar 3;4:4260. doi: 10.1038/srep04260

Figure 4. IL-6 delayed the degradation of BTB-constituent proteins.

Figure 4

(a) Immunoblotting for quantification of Occludin, JAM-a, and N-Cadherin that remained non-degraded after 50 pg/ml IL-6 treatment for 0 min, 30 min, 1 h and 3 h. β-Actin served as the loading control. All gels had been run under the same experimental conditions. (b) Bar plots summarizing relative Occludin, JAM-a, and N-Cadherin results from several independent experiments after normalizing each data point against its corresponding actin time point and then against its corresponding control at 0 min. Control in both experimental groups was arbitrarily set at 1. Data points represent median ± AD (n = 3). *P < 0.05, vs. control.