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. 2014 Jan 27;6(2):448–475. doi: 10.3390/v6020448

Figure 1.

Figure 1

Western blot analysis of sera from three “naturally seropositive” commercially purchased outbred Hartley guinea pigs. (a) Western analysis using a pooled, high-titer polyclonal anti-GPCMV antisera from guinea pigs immunized with adjuvanted viral particles [30] or sera from three “natural seropositive” guinea pigs (GP1, GP2, GP3) obtained from a commercial supplier, using sucrose gradient-purified virions from 22122 strain as target antigen. Two independently derived, monospecific anti-GPCMV gB antibodies (moab 29-29 [31] (gB1) and moab IE321 (gB2)) are included as controls. (b) Photomicrographs comparing CPE of low-passage (P2) CIDMTR virus (right panel) to GPCMV strain 22122 (left panel) following infection of GPL cells. CIDMTR plaques are more rounded, with elliptical appearance, compared to the more spindle-like morphology of 22122-infected fibroblasts. Plaques photographed at 60× magnification.