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. 2014 Jan 27;6(2):448–475. doi: 10.3390/v6020448

Figure 4.

Figure 4

RT-PCR mapping of CIDMTR strain and 22122 strain splice sites. (a) Cartoon representation of the IE1/2 gene locus illustrating positions of primer pairs used for RT‑PCR. Introns are indicated by straight lines and exons are blue boxes, although exon 1 and 2 are non-coding. (b) Results of RT-PCR reactions e4-1, e5-2, and GAPDH (control) using RNA from uninfected cells or cells infected with 22122 or CIDMTR. (c) RT-PCR consensus sequence of CIDMTR strain. Exon junctions are highlighted in blue. The first gray highlighted sequence is an ATA codon; this is an ATG codon and the putative start codon for the 22122 IE1/2 proteins, but is not conserved in the CIDMTR sequence in tissue culture passaged virus. The second gray highlighted sequence may therefore represent the putative start codon for CIDMTR TC IE1/2.