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. 2014 Jan 27;6(2):448–475. doi: 10.3390/v6020448

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

5' RACE analysis of CIDMTR IE region. (a) Schematic of IE gene indicating primers pair used in RACE. (b) nPCR gels for 22122 and CIDMTR using RNA purified under IE conditions. The CIDMTR RACE product is 734 nt compared to 729 nt for the 22122 strain, explaining its slightly higher migration by gel electrophoresis. (c) RACE consensus sequence of CIDMTR IE. Exon junctions highlighted in blue. First gray highlighted sequence is co-linear to the start codon for 22122; second highlighted sequence represents putative start codon for CIDMTR IE1/2.