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SDS-PAGE (A) and Western blot analysis (B–D) of purified full-length PUUV-Gc protein (lane 1) with an ubiquitin-specific polyclonal antibody (B) and the MAbs of clones #1F5 (C) and #7A12 (D) raised against yeast-expressed, His-tagged entire PUUV-Kazan Gc protein. Purified PUUV Vranica/Hällnäs N protein (lane 2) was used as a negative control. Lanes M, M * and M **, Prestained Protein Molecular Weight Markers (UAB, “Thermo Fisher Scientific Baltics”, Vilnius, Lithuania).
