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Analysis of the specificity of the MAb #10B8 by Western blot: (A) Coomassie blue-stained SDS-PAGE; (B) Western blot with MAb #10B8. Lane M, Prestained Protein Molecular Weight Marker (UAB “Thermo Fisher Scientific Baltics”); lane 1, HaPyVP1; lane 2, VP1-PUUV-Gc99#1 VLPs; lane 3, VP1-PUUV-Gc99#4 VLPs; lane 4, PUUV Kazan N protein; lane 5, Dobrava Slovenia N protein; lane 6, negative control sample from whole crude lysate of S. cerevisiae cells transformed with the “mock” vector pFX7.
