Table 1. Immunisation scheme.
| Group | VLP antigen dose | Horse | Age | Sex | Breed |
|---|---|---|---|---|---|
| 1 | Adjuvant only | 1 | 24 | G | WB |
| (Al(OH)3, 250 μg) | 2 | 8 | M | SB | |
| 3 | 18 | G | SB | ||
| 2 | 50 μg | 4 | 7 | M | SB |
| 5 | 7 | G | SB | ||
| 6 | 17 | G | WB | ||
| 7 | 19 | G | TB | ||
| 3 | 100 μg | 8 | 17 | G | WB |
| 9 | 18 | G | WB | ||
| 10 | 20 | M | WB | ||
| 11 | 20 | M | WB | ||
| 4 | 150 μg | 12 | 4 | G | SB |
| 13 | 4 | G | SB | ||
| 14 | 19 | G | WB | ||
| 15 | 30 | G | WB |
Incremental doses of antigen as indicated were injected deep intramuscularly into the left side of the neck (Groups 2, 3, 4). Three control horses (Group 1) received alum hydroxide adjuvant only. Age, gender and breed are indicated (G: gelding, M: mare; SB: Standardbred trotter, TB: Thoroughbred, WB: Warmblood).