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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Stroke. 2014 Feb 4;45(3):857–864. doi: 10.1161/STROKEAHA.113.004100

Figure 6. PD-L1 is critical for Treg-afforded neuroprotection after MCAO.

Figure 6

(A-B) Tregs were pre-incubated with PD-L1 neutralizing Abs or isotype control IgG at 20 μg/mL and then adoptively transferred into MCAO animals after 2 h of reperfusion. (A) Representative TTC staining of brain infarct and quantification of infarct volume at 3 days after MCAO. n=7/group. (B) Neurological deficit scores over 3 days after MCAO. n=7/group. (C-D) Tregs were prepared from PD-L1 knockout mice and then adoptively transferred into MCAO animals after 2 h of reperfusion. (C) Representative MAP2 staining of brain infarct and quantification of infarct volume at 3 days after MCAO. n=5/group. (D) Neurological deficits scores over 3 days after MCAO. n=5/group. *P<0.05, **P<0.01, ns: not significant. Sp = splenocytes.