Figure 1. Df-treated Pla2g5-null mice have reduced expression of molecules associate with alternative activation of Mϕ.

(A, B) Sections from the lungs of WT and Pla2g5-null mice treated with saline (Ai,iii) or Df (Aii,iv; Bi,ii) were stained for Relm-α (red) and nuclei (blue). (A) Original magnification × 20, bar 50 µm. (B) High magnification of regions depicted in A. Arrows point to Relm-α staining on epithelium of WT (large arrow) and Pla2g5-null (small arrow) mice. Large arrowhead points to a Mϕ, small arrowhead points to an eosinophil. (C) Forward (FSC) and side scatter (SSC) characteristics (left panels) and CD68 gating (right panels) of CD68^enr/Df-lung-cells from WT and Pla2g5-null mice. (D) Net (isotype control subtracted) mean fluorescence intensity (MFI) of Dectin1 and Relm-α on gated CD68⁺ WT (filled bars) and Pla2g5-null (open bars) cells. (E) Expression of mRNA encoding alternatively (CCL22, Arg-1, MMP-12, CCL17, YM1, and CCL11) and classically activated (CXCL10 and iNOS) Mϕ markers measured by qPCR. Data are expressed as ratio of the indicated mRNA expression relative to GAPDH. (A, B) Images are from one representative mouse per group from one of two experiments. (C) The lung cells are pooled from 7–9 mice per group, and representative plots are from one of six experiments. (D and E) Values are mean ± SEM of four to six independent experiments. *, p <0.05; #, p<0.02