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. Author manuscript; available in PMC: 2015 Feb 4.
Published in final edited form as: Structure. 2013 Dec 19;22(2):230–237. doi: 10.1016/j.str.2013.11.007

Figure 5. Native mass-spectrometry quantification of protease packaging and cleavage efficiency.

Figure 5

(top) The mass spectrum obtained for the protease-free procapsid indicates a mass of 17.9 +/- 0.004 MDa while the theoretical one is 17.7MDa (mass accuracy of 1%). (middle) The mass spectrum obtained for the protease-containing procapsid is noisier and yields a mass estimate of 21.4 ± 0.34 MDa. (bottom) Mass spectrum of Head-2 reveals three distributions with a mass of approximately 12.9 MDa. The masses are consistent with a highly efficient maturation cleavage but clearly reveal some residual material in the Head-2 particles. (insets) Zoom-in of the indicated m/z region to reveal the fine structures on the signal.