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. Author manuscript; available in PMC: 2014 Mar 3.
Published in final edited form as: Eur J Immunol. 2010 Dec 9;41(1):172–181. doi: 10.1002/eji.201040385

Figure 5. (A) IL-18 is crucial for Borrelia induced IFN-γ but not IL-33 production.

Figure 5

(A, B) 5 × 106 /well spleen cells of 5 WT C57BL/6 mice and 5 IL-18 knock-out mice were incubated with 1 × 106 spirochetes/ml B. burgdorferi or B. afzelii or medium alone for 5 days. Supernatants were collected for measurement of (A) IFN-γ or (B) IL-17 by ELISA. Data are mean ± SEM in pg/ml; 5 mice per group *p<0.05 two-tailed Mann-Whitney U test. (C) Spleen cells (5 × 106 /well) of 3 wild-type Balb/c mice were cultured for 5 days in RPMI alone or with 1 × 106/mL B. burgdorferi in the presence/absence of 10 μg/ml IL-33 antibody or 10 μg/ml control goat IgG antibody. Supernatants were collected and IL-17 was measured by ELISA. Data are mean ± SEM; pg/ml; 3 animals per group.