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. Author manuscript; available in PMC: 2014 Mar 3.
Published in final edited form as: Exp Biol Med (Maywood). 2012 Sep 3;237(9):1101–1109. doi: 10.1258/ebm.2012.012043

Fig. 1. Bone formation is greatly diminished in sleep-restricted rats.

Fig. 1

Representative micrograph (400×) of metaphyseal femur stained with Masson’s Trichrome in an ambulation control (A) and a sleep-restricted (B) rat. Osteoid is stained blue. Osteoblasts are basophilic bone-lining cells, shown here as either plump (A), considered activated, or flattened (B), considered quiescent. A subset of osteoblasts is designated by arrows. The proportion of metaphyseal bone perimeter lined by osteoid in individual ambulation control (○) and sleep-restricted (●) rats is shown by dot plot (C). Group averages are indicated by a horizontal bar. The group comparison is statistically significant at P = 0.002.

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